示例文件：火山图示例文件

1. 数据整理

文件格式及数据内容详见说明部分。

2. 文件输入与参数选择

点击选择文件，载入绘图数据后按需选择参数即可，参数说明详见说明部分。参数填写完毕后，点击提交即可。

3. 结果输出

在“项目总览”中查看进程及结果，点击“预览”可在线查看火山图结果，点击“下载”可输出pdf及png格式的火山图到本地。

Q1. 数据文件名称有没有要求？

OmicShare当前支持文件命名为中文、英文、符号仅支持英文点号“.”及下划线"_"。

不支持空格及其他特殊符号。

Q2. 提交时报错常见问题：

1.提交时显示X行X列空行/无数据，请先自查表格中是否存在空格或空行,需要删掉。

2.提交时显示列数只有1列，但表格数据不止1列：列间需要用分隔符隔开，先行检查文件是否用了分隔符。

Q3. 为什么提示x轴y轴数据不能来自同一列？

在提交分析前，需要选择log2(FC)、P值或FDR值所在列。没有填写所在列则会如上提示。

Q4.为什么任务出错？

如果数据文件没有问题，可以检查是否log2(FC)、P值或FDR值所在列选错。

注意：“第_列作为x/y轴的值”这里需要填写阿拉伯数字！

例：

比如在我的数据中，log2(FC)在2列，P value在3列，这里相应填上2、3就可以了，而切勿输入B列、C列！

Q5. 如何对火山图进行调整（文字/图形样式调整）？如何在图中标注基因？

在线调整及标注基因功能，需要使用动态火山图工具。

点击跳转至动态火山图工具>>

Q6. 结果页面窗口有问题，图表加载不出来怎么办？

尝试用谷歌浏览器登录OmicShare查看结果文件，部分浏览器可能不兼容。

引用OmicShare Tools的参考文献为：

案例1

发表期刊：Ecotoxicology and Environmental Safety

影响因子：6.8

发表时间：2022

Fig. 4. b&c, Volcano plots of protein quantifications in CK group (b) and AZA exposure group (c), respectively, and the logarithmic fold changes of iTRAQ quantification intensities were plotted by the negative decadic logarithm of the p value. The number of up-/down-regulated DAPs was obtained by comparing the protein abundance in adult ovary with that in pupal ovary in S. litura under normal and AZA exposure condition, respectively.

引用方式：

Volcano plots were performed using the OmicShare tools (https://www.omicshare.com/tools) with default settings.

参考文献：

Sun R, Xu Y, Liu J, et al. Proteomic profiling for ovarian development and azadirachtin exposure in Spodoptera litura during metamorphosis from pupae to adults[J]. Ecotoxicology and Environmental Safety, 2022, 237: 113548.

案例2

发表期刊：Frontiers in Molecular Neuroscience

影响因子：4.8

发表时间：2022

FIGURE 5. A visualization of the transcriptome data analysis. The volcano map (A) and heatmap (B) of the gene expression. The red gene is upregulated and the green gene is downregulated. |log2 (FC)| >1 is considered to be significant.

引用方式：

We drew the volcano map and the heatmap of the DEGs using the OmicShare tools（www.omicshare.com/tools）.

参考文献：

Li M, Liu F, Hao X, et al. Rare KCND3 Loss-of-Function mutation associated with the SCA19/22[J]. Frontiers in Molecular Neuroscience, 2022, 15: 919199.

案例3：

发表期刊：Journal of agricultural and food chemistry

影响因子：6.1

发表时间：2020

Figure 5. Characterization of PCH, FCH, and P-FCH in terms of amino acid and peptide profiles. (E) Volcano plot of peptides in PFCH vs PCH (red dots denote upregulation, and green dots denote downregulation). (F) Volcano plot of peptides in P-FCH vs FCH (red dots denote upregulation, and green dots denote downregulation).

引用方式：

The distribution of peptides in each hydrolysate was analyzed by an UpSet Venn diagram (http://www.omicshare.com/ tools). Variation analysis of the peak areas in common peptides was shown by the volcano plot (http://www.omicshare.com/tools).

参考文献：

Wang C, Zheng L, Su G, et al. Evaluation and exploration of potentially bioactive peptides in casein hydrolysates against liver oxidative damage in STZ/HFD-induced diabetic rats[J]. Journal of agricultural and food chemistry, 2020, 68(8): 2393-2405.

案例4：

发表期刊：Infection and Drug Resistance

影响因子：3.9

发表时间：2022

Figure 2 Data quality control and differential screening. (D) volcano plot showing up-regulated (purple) and down-regulated (green) proteins screened by differential analysis, with the logarithm of the difference multiplicity (log2) as the horizontal coordinate and the negative logarithm of the p-value (log10) as the vertical coordinate.

引用方式：

Differentially expressed proteins were screened using the OmicShare online platform (https://www.omicshare.com/) with the screening criteria: p<0.05 and |FC|≥2.0. Transcriptomic and proteomic data are submitted to OmicShare platform to construct a nine-quadrant map.

参考文献：

Wang C, Li Y, Li S, et al. Proteomics combined with RNA sequencing to screen biomarkers of sepsis[J]. Infection and Drug Resistance, 2022: 5575-5587.