1. 切换任务

下拉框内选择相应任务编号，可以切换任务查看。由于火山图基因数目较多，切换时需稍作等待；

2. 基本设置更改

在基本设置中，可对文本样式、坐标轴系、图例相关参数进行修改；

3. 图形设置更改

在图形设置中，可对图形相关参数进行修改；

4. 自定义标签添加

使用套索模式，或在下方表格中勾选想要展示的id标签，即可在火山图中显示；

在图形设置——已选中散点模块中，可对选中的散点、文本标签、标签线的颜色、大小等进行修改。

Q1. 数据文件名称有没有要求？

OmicShare当前支持文件命名为中文、英文、符号仅支持英文点号“.”及下划线"_"。

不支持空格及其他特殊符号。

Q2. 提交时报错常见问题：

1.提交时显示X行X列空行/无数据，请先自查表格中是否存在空格或空行,需要删掉。

2.提交时显示列数只有1列，但表格数据不止1列：列间需要用分隔符隔开，先行检查文件是否用了分隔符。

Q3. 为什么提示x轴y轴数据不能来自同一列？

在提交分析前，需要选择log2(FC)、P值或FDR值所在列。没有填写所在列则会如上提示。

Q4.为什么任务出错？

如果数据文件没有问题，可以检查是否log2(FC)、P值或FDR值所在列选错。

注意：“第_列作为x/y轴的值”这里需要填写阿拉伯数字！

例：

比如在我的数据中，log2(FC)在2列，P value在3列，这里相应填上2、3就可以了，而切勿输入B列、C列！

Q5. 如何对火山图进行调整（文字/图形样式调整）？

使用动态火山图工具，在任务完成后，点击“跳转查看”小图标即可跳转界面修改。

Q6. 如何在火山图中标注基因？

图中标注基因的功能需要使用动态火山图工具。

在动态调整窗口下拉，可以看到结果输入表，①选择需要搜索的列；②在筛选框中输入需要标注的基因名，即可将基因在火山图中标注显示。

引用OmicShare Tools的参考文献为：

案例1

发表期刊：Ecotoxicology and Environmental Safety

影响因子：6.8

发表时间：2022

Fig. 4. b&c, Volcano plots of protein quantifications in CK group (b) and AZA exposure group (c), respectively, and the logarithmic fold changes of iTRAQ quantification intensities were plotted by the negative decadic logarithm of the p value. The number of up-/down-regulated DAPs was obtained by comparing the protein abundance in adult ovary with that in pupal ovary in S. litura under normal and AZA exposure condition, respectively.

引用方式：

Volcano plots were performed using the OmicShare tools (https://www.omicshare.com/tools) with default settings.

参考文献：

Sun R, Xu Y, Liu J, et al. Proteomic profiling for ovarian development and azadirachtin exposure in Spodoptera litura during metamorphosis from pupae to adults[J]. Ecotoxicology and Environmental Safety, 2022, 237: 113548.

案例2

发表期刊：Frontiers in Molecular Neuroscience

影响因子：4.8

发表时间：2022

FIGURE 5. A visualization of the transcriptome data analysis. The volcano map (A) and heatmap (B) of the gene expression. The red gene is upregulated and the green gene is downregulated. |log2 (FC)| >1 is considered to be significant.

引用方式：

We drew the volcano map and the heatmap of the DEGs using the OmicShare tools（www.omicshare.com/tools）.

参考文献：

Li M, Liu F, Hao X, et al. Rare KCND3 Loss-of-Function mutation associated with the SCA19/22[J]. Frontiers in Molecular Neuroscience, 2022, 15: 919199.

案例3：

发表期刊：Journal of agricultural and food chemistry

影响因子：6.1

发表时间：2020

Figure 5. Characterization of PCH, FCH, and P-FCH in terms of amino acid and peptide profiles. (E) Volcano plot of peptides in PFCH vs PCH (red dots denote upregulation, and green dots denote downregulation). (F) Volcano plot of peptides in P-FCH vs FCH (red dots denote upregulation, and green dots denote downregulation).

引用方式：

The distribution of peptides in each hydrolysate was analyzed by an UpSet Venn diagram (http://www.omicshare.com/ tools). Variation analysis of the peak areas in common peptides was shown by the volcano plot (http://www.omicshare.com/tools).

参考文献：

Wang C, Zheng L, Su G, et al. Evaluation and exploration of potentially bioactive peptides in casein hydrolysates against liver oxidative damage in STZ/HFD-induced diabetic rats[J]. Journal of agricultural and food chemistry, 2020, 68(8): 2393-2405.

案例4：

发表期刊：Infection and Drug Resistance

影响因子：3.9

发表时间：2022

Figure 2 Data quality control and differential screening. (D) volcano plot showing up-regulated (purple) and down-regulated (green) proteins screened by differential analysis, with the logarithm of the difference multiplicity (log2) as the horizontal coordinate and the negative logarithm of the p-value (log10) as the vertical coordinate.

引用方式：

Differentially expressed proteins were screened using the OmicShare online platform (https://www.omicshare.com/) with the screening criteria: p<0.05 and |FC|≥2.0. Transcriptomic and proteomic data are submitted to OmicShare platform to construct a nine-quadrant map.

参考文献：

Wang C, Li Y, Li S, et al. Proteomics combined with RNA sequencing to screen biomarkers of sepsis[J]. Infection and Drug Resistance, 2022: 5575-5587.