report_heatnetwork


Select a file *  Example
Select a file *  Example
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1. Global modification

2.Grid modification

3.Line modification

4.Legend modification

5. Preview and download graphics

I. Functions

 

Applied to multi-omics association analysis, it can display the correlation results within omics (heat map) and between omics (network map). Among them, the intraomics correlation heat map is used to display the correlation coefficients between every two elements (environmental factors, metabolites, traits, etc.); The inter-omics correlation network diagram can display the correlation between 16S-OTU, metagenomic-species, gene and other abundance information and each element of the correlation data file.

Click to view the interpretation of the graphic meaning: detailed principles of the graphic

 

Examples of application scenarios: such as microorganisms and environmental factors, genomes and metabolites, transcriptomes and traits, etc.

Scenario 1: Microbe + environmental factors

Heat map: a heat map of the correlation between two environmental factors;

 

Network diagram: the correlation between amplicons, metagenomic abundance or diversity index and each environmental factor.

Scenario 2: Transcriptome + Metabolites

Heat map: a heat map of the correlation between metabolites;

Network diagram: Correlation between transcript abundance and each metabolite.

Scenario 3: Transcriptome + Phenotype

Heat map: a heat map of the correlation between phenotypic traits;

 

Network diagram: The correlation between gene abundance modules and each trait.

 

2. Scope of application

 

Suitable for 16S, transcriptome, metabolome, proteinome and other data suitable for drawing heat maps and network maps.

 

3. Enter

 

1) Correlation data table: The correlation or significance table between every two columns of elements in the omics input here is used to draw a heat map;

2) Associated data table: The association table between omics entered here is used to draw network diagrams;

3) Input file format: The file supports txt (tab delimited) text files, csv (comma delimited) text files, and the exclusive xlsx format for Excel. It also supports the xls (Excel 97-2003) format of the old version of Excel. The file name is only Can be composed of letters, numbers, and underscores.

 

4) Example of input file:

Examples of heat map files:

 

Examples of network diagram files:

4. Dynamic parameters

 

a. Grid modification: including basic modifications such as grid width, height, shape (can be modified to a circle), grid boundary (checked by default), etc.

b. Line modification: Two choices of single/gradual change are given, and the color and thickness of the line can be modified according to the Pvalue and the correlation coefficient.

 

c. Legend modification: The legend accuracy, number of scales, legend height, and legend width of the heat map can be modified, and the line legend can be displayed/hide at the same time.

 

 

5. Result output-graphical example

 

 

Interpretation of the results:

 

1) In the heat map on the right, the horizontal and vertical axes respectively represent various environmental factors that have a response/interaction relationship with the community. The color of each square in the heat map indicates the positive and negative correlation coefficients between environmental factors. The size indicates the absolute value of the correlation coefficient.

 

 

2) The left side is the data of various microbial communities obtained by 16s and ITS sequencing, and these data are correlated with the data of various environmental factors one by one through the connection. The thickness of the line indicates the strength of the correlation, and the color of the line indicates the degree of significanc.

 

 

 

 

Results Display      (Click " project ID" to view different analysis results)