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五周年,102个工具都降价了

report_enrich_circle




choosefile   example








Input the sample file: sample_table

Parameter selection: the first 20 lines of data drawing 、 P value drawing

Output graphics:

1.global modification

2.Basic color matching modification


3. Interval modification and graphic result download




1. Function:

Use the enrichment analysis result table to draw the enrichment circle map.

2. Input file:

Input the matrix table file, where:

Column 1: ID, for example, enter the ID information of GO term.

Column 2: Classification, the classification name corresponding to this ID, and the function legends in the graph are displayed according to the information in this column.

Column 3: all, which indicates the total number of genes identified by this sample in this classification, that is, background genes.

Column 4: P value /Q value, plot based on p value or q value, and enter only one.

Column 5 and 6: There are two formats for input. When distinguishing up-down genes, the numbers of up-down genes and down-down genes are input in column 5 and 6 respectively. When there is no distinction between up-regulation and down-regulation, you only need to input the total number of differential genes in the fifth column.

Example file 1 (6 columns in total, data table for distinguishing up-down regulation genes):

Example document 2 (5 columns in total, no distinction between up and down-regulated genes)


(Note: in the final drawing, the rich factor = (5 columns +6 columns) /3 columns)

Input file type: the input file must be a tab-separated. txt file with header. The table name consists of letters, numbers and underscores, and no suffix name is allowed. You can choose to open the data in excel and save it as "Text File (tab delimited) (*.txt)"

3. Parameter selection:

A. parameter: select p value or q value for drawing (this information will be displayed in the legend)

B. Number of lines for drawing: select the number of data lines for drawing from the drop-down list. It is recommended to use no more than 25 lines of data for drawing. Too many lines of drawing may lead to overlapping words and affect the perception.

3. Graphics adjustment parameters

Global modification:

A. font/name size: adjust the font size of the corresponding position

B. Basic legend: check the display position of basic legend

C. check the display: function legend, significance legend and function name

D. Self-adaptation: self-adaptation of scale outside the circle/input scale range, self-adaptation of column diagram inside the circle/input value range

Basic color matching:

A. name color: modify the first ID text color

B. Up-down gene color matching: modifying up-down gene color matching

C. Functional color matching: adjustable color matching of each function

Interval color matching:

The value of significance value (-log10(P value)/-log10(Q value)) interval can be adjusted, and color matching can be selected for each sub-interval.

The result diagram is as follows:

1. Distinguish the up-down gene pattern:


2. Do not distinguish the up-down gene pattern:



Module function

Show the results of enrichment analysis by using enrichment circle diagram.

Graphic interpretation

There are four laps from outside to inside.

1.The first circle: the classification of enrichment, and the coordinate scale of gene number outside the circle. Different colors represent different classifications;

2.The second circle: the number of the classification in the background gene and the Q value or P value. The more genes, the longer the bar, and the smaller the value, the redder the color

3.The third circle: the bar chart of up-down gene ratio, deep purple means up-down gene ratio, light purple means down-down gene ratio; Specific values are shown below; When the number of input differential genes is only one column (no distinction between up and down), the third circle shows the total number of foreground genes;

4.Circle 4: RichFactor value of each classification (the number of foreground genes divided by the number of background genes in this classification), and the background auxiliary line represents 0.1 in each cell.




Results Display      (Click " task ID" to view different analysis results)