Input：
paste or upload a nucleic or protein sequence in FASTA format.
Output:
1. alignment result table
2. alignment statistic pie chart ( alignment number pie chart and E-value distribution pie chart)
3.Pathway enrichment analysis table and chart( table of enrichment analysis result and pathway enrichment histogram )
Output:
1. alignment result table
Headers:
Query_id the ID of the query sequence
Subject_id the ID of the subject sequence
Organism Species(OS) the species name of the subject sequence
Gene Name(GN) the gene name of the subject sequence
Identity(%) the alignment similarity (percentage)
Align_length alignment length
Mis_match number of mismatch
Gap number of Gap
Query_start the start site of the aligned query sequence
Query_end the end site of the aligned query sequence
Subject_start the start site of the aligned subject sequence
Subject_end the end site of the aligned subject sequence
E_value E value of alignment (the smaller the E value, the more the reliability)
Score the score of alignment (the higher the score, the more the reliability)
Subject_annotation description of GO subject sequence
2. alignment number pie chart
We count up the aligned sequences number and show the result with a pie chart.
E-value distribution pie chart
E value is the expected value of the alignment. The smaller the E value, the more the reliability. We divided the E value into five ranges and show the number with a pie chart.
3. Pathway enrichment analysis
table of enrichment analysis result
Form content including Pathway, Pathway ID, Number of Gene, Gene ID, KO ID.
pathway enrichment bubble chart